| 主权项 |
1. A method for determining the concentration of calprotectin in a sample comprising the following steps:
i) coating a purified S100A9 or a peptide thereof onto a solid phase, ii) reacting the S100A9 or peptide coated on the solid phase in step i) with a) a sample containing calprotectin and b) a labelled anti-S 100A9 antibody, iii) washing of the solid phase after the reaction of any calprotectin in the sample and the labelled anti S100A9 antibody to remove unbound anti-S 100A9 antibody from the solid phase, iv) determining the quantity of labelled anti-S 100A9 antibody bound to the solid phase to determine the concentration of calprotectin in the sample; wherein the quantity of S100A9 polypeptide coated onto the solid phase and the quantity of labelled anti-S 100A9 antibody are chosen such that the S100A9 or peptide thereof coated onto the solid phase and any calprotectin in the sample compete for the labelled anti-S100A9 antibody such that the quantity of labelled anti-S100A9 antibody bound to the solid support is related inversely to the concentration of calprotectin in the sample. |
