ELECTROBLOT TRANSFER BUFFER

摘要

A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.

主权项

1. An electroblot transfer method comprising
assembling a membrane and a gel, the gel containing separated proteins, peptides, and/or nucleic acids, the membrane and the gel sandwiched between at least two pieces of absorbent material, the absorbent material saturated with a transfer buffer comprising N-(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine (tricine), tris(hydroxymethyl)aminomethane (tris), glycine, and optionally ethylenediaminetetraacetic acid (EDTA); and applying a current to effect reduced resistance in transfer of the separated proteins, polypeptides, and/or nucleic acids from the gel to the membrane.