TEVL CHIMERIC ENDONUCLEASE AND THEIR PREFERENTIAL CLEAVAGE SITES

摘要

The present invention relates to a method to cleave target nucleic acid sequence by the catalytic domain of a GIY-YIG homing endonucleases l-Tevl. More precisely, the invention relates to the deciphering of new preferential l-Tevl cleavage sites for efficient and specific cleavage activity. The invention concerns a method for the generation of TevI specific chimeric endonucleases to target nucleic acid sequence including such cleavage sites and methods of using same for gene editing.