发明名称 |
CONTINUOUS EXTENSION AND DEBLOCKING IN REACTIONS FOR NUCLEIC ACID SYNTHESIS AND SEQUENCING |
摘要 |
A reaction mixture including (a) a nucleic acid having a primer hybridized to a template, (b) nucleotide analogs, wherein the nucleotide analogs have a blocking moiety; (c) a polymerase that is capable of forming an extended primer by adding the nucleotide analogs to the primer, and (d) a deblocking agent that is capable of removing the blocking moiety from the extended primer. Also provided is a method of synthesizing a polynucleotide including sequentially adding a plurality of the different nucleotides analogs to the nucleic acid via several reaction cycles in the reaction mixture, wherein each reaction cycle includes (i) the polymerase adding a nucleotide analog to the nucleic acid to form a transient nucleic acid species comprising a blocking moiety, and (ii) the deblocking agent modifying the transient nucleic acid species to remove the blocking moiety. |
申请公布号 |
EP2761026(A1) |
申请公布日期 |
2014.08.06 |
申请号 |
EP20120777938 |
申请日期 |
2012.09.28 |
申请人 |
ILLUMINA, INC. |
发明人 |
MEULEMAN, WOUTER;CRESSINA, ELENA;SMITH, GEOFFREY PAUL;JACKSON, ROSAMOND;LIU, ZIAOHAI |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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