| 摘要 |
The invention relates to a rapid analysis method for the label-free identification and detection of Gram + and Gram - bacteria and/or viruses that may or may not be antibiotic resistant, using audio-video discs (CD, DVD, BD, Lightscribe, Superdisk, etc.) as a support for immobilising specific molecular receptors of biomolecules present on the surface of the bacteria and/or viruses, using a disc player/recorder (CD, DVD, BD, Lightscribe, etc.) as a detector. The invention comprises three main processes: resuspension of the viruses and/or bacteria, incubation on the disc, and label-free detection using a compact disc player/recorder (CD, DVD, BD, etc.). The invention has been designed to identify receptors or bioreceptors immobilised separately on the polycarbonate face of the disc in known ordered positions, forming a micro-array. The specific bioreceptors of biomolecules present on the surface the viruses and/or bacteria can be, for example, proteins, lipids and nucleic acids. |
