Method and device for reading an emulsion

摘要

A method for reading an emulsion (3) including droplets and a continuous phase surrounding the droplets, the method includes:
two-dimensional scanning of the emulsion (3), andconstruction of a two-dimensional image of the emulsion (3) based on the scanning. Preferably, the droplets do not move during scanning, for example by solidifying the continuous phase or by using a two-dimensional compact or semi-compact network of droplets. The method can further include time-based monitoring of a chemical or biological reaction taking place in at least one of the droplets. A device implementing this method is also described. The method is applicable for the detection and/or sorting of microdroplets performing the role of microreactors or containing specific cells or molecules, in fields such as gene expression or diagnosis.

主权项

1. A method for reading an emulsion comprising droplets and a continuous phase which surrounds the droplets, said method comprising:
two-dimensional scanning the emulsion with an optical system, the emulsion being maintained during the scanning at a temperature below the melting point of the continuous phase; and constructing a two-dimensional image of the emulsion based on said scanning.