| 发明名称 |
Hydrophobic interaction chromatography method |
| 摘要 |
Herein is reported a method for purifying a polypeptide comprising a histidine-tag comprising the steps of i) applying a solution comprising the polypeptide with a histidine-tag to a hydrophobic interaction chromatography material, and ii) recovering the polypeptide comprising a histidine-tag with a solution comprising imidazole or an imidazole-derivative and thereby purifying the polypeptide comprising a histidine-tag, wherein the solution comprising the polypeptide applied to the hydrophobic interaction chromatography material is free of imidazole or an imidazole-derivative and the polypeptide adsorbed to the hydrophobic interaction chromatography material is recovered with a solution comprising imidazole or an imidazole-derivative. |
| 申请公布号 |
US8796419(B2) |
申请公布日期 |
2014.08.05 |
| 申请号 |
US201113697406 |
申请日期 |
2011.05.17 |
| 申请人 |
Hoffmann-La Roche Inc. |
发明人 |
Falkenstein Roberto;Fuehrler Nicole;Smida Maria |
| 分类号 |
C07K1/20;C12P21/00 |
主分类号 |
C07K1/20 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for purifying a polypeptide comprising a histidine-tag comprising the following steps:
applying a solution comprising the polypeptide with a histidine-tag to a hydrophobic interaction chromatography material, and recovering the polypeptide comprising a histidine-tag with a solution comprising imidazole or an imidazole-derivative from the hydrophobic interaction chromatography material and thereby purifying the polypeptide comprising a histidine-tag. |
| 地址 |
Nutley NJ US |
