METHOD AND APPARATUS FOR PERFORMING QUALITATIVE AND QUANTITATIVE ANALYSIS OF BURN EXTENT AND SEVERITY USING SPATIALLY STRUCTURED ILLUMINATION

摘要

Frequent monitoring of early-stage burns is necessary for deciding optimal treatment and management. Superficial-partial thickness and deep-partial thickness burns, while visually similar, differ dramatically in terms of clinical treatment and are known to progress in severity over time. The disclosed method uses spatial frequency domain imaging (SFDI) far noninvasively mapping quantitative changes in chromophore and optical properties that may be an indicative of burn wound severity. A controlled protocol of graded burn severity is developed and applied to 17 rats. SFDI data is acquired at multiple near-infrared wavelengths over a course of 3 h. Burn severity is verified using hematoxylin and eosin histology. Changes in water concentration (edema), deoxygenated hemoglobin concentration, and optical scattering (tissue denaturation) are statistically significant measures, which are used to differentiate superficial partial-thickness burns from deep-partial thickness burns.

主权项

1. A method using spatial frequency domain imaging (SFDI) for quantitative noninvasive, noncontact assessment of severity of burn injury to tissue in vivo comprising:
performing wide-field quantitative mapping of tissue optical properties including pixel-by-pixel demodulation and fitting of spatial frequency data performed with multispectral imaging to extract the local absorption and reduced scattering optical coefficients; performing quantitative analysis of burn injury of tissue by separately analyzing absorption spectra at each pixel to yield a spatial map of blood oxygenation, water concentration, optical scattering changes or a combination thereof; and classifying the severity of the burn injury to tissue according to the depth of the injury.