发明名称 |
USE OF RNA REMOVAL TO INITIATE PROTEIN AGGREGATION |
摘要 |
The present invention provides the use of RNA removal to initiate protein aggregation of a plurality of proteins in a cell or cell lysate. This may be used to create an in vitro model of a disease, such as a neurodegenerative disease. The present invention also provides a method for determining the efficacy of a potential anti-protein aggregation agent comprising the following steps: i) using RNA removal to initiate the aggregation of a protein in a cell or cell lysate, ii) treating the cell or cell lysate with the potential anti-protein aggregation agent before, after or during RNA removal; and iii) comparing protein aggregation in equivalent samples with and without step ii) treatment in which a decrease in protein aggregation associated with step ii) treatment indicates that the potential anti-protein aggregation agent is effective in preventing and/or reversing protein aggregation. |
申请公布号 |
WO2014114937(A1) |
申请公布日期 |
2014.07.31 |
申请号 |
WO2014GB50177 |
申请日期 |
2014.01.23 |
申请人 |
QUEEN MARY UNIVERSITY OF LONDON |
发明人 |
AARUM, JOHAN;SHEER, DENISE |
分类号 |
C12Q1/34 |
主分类号 |
C12Q1/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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