PREPARATION OF REACTION CHAMBERS WITH DRIED PROTEINS

摘要

The present invention relates to a method of applying a spot of an unlabelled biomolecule, e.g. antibody or protein antigen, to a surface of a reaction chamber of a diagnostic assay. This method comprising the steps of applying to the surface of the reaction chamber a solution comprising a sugar and comprising a non-labelled biomolecule, e.g. antibody or protein antigen, and allowing the solution to dry. In this method the biomolecule is in a concentration sufficient to saturate the binding places for a protein on the surface where the solution has been applied to. The present invention relates to a reaction chamber of a diagnostic device for performing a biomolecule, e.g. antibody or protein antigen, based detection assay. Herein,the reaction chamber comprises a detection region with one or more spots of an unlabelled biomoleculespots bound to the detection region. The one or more spots have a diameter of between 0.1 to 0.5 mm. The spot comprises a sugar and a protein and comprises between 0.01 and 0.5 ng biomolecule.

主权项

1. A method of applying a spot with a diameter of between 100 to 500 μm of an unlabelled biomolecule to a surface of a reaction chamber of a diagnostic assay from a sample, the method comprising the steps of:
applying to the surface of the reaction chamber a solution comprising a sugar and comprising a non-labelled biomolecule, and allowing the solution to dry,wherein the biomolecule is in a concentration just sufficient to saturate the binding places for a protein on the surface where the solution has been applied to such that the reaction chamber with the dried biomolecule undergoes no washing steps prior to the application of a determined sample in said reaction chamber, to remove an excess of unbound biomolecules.