Recombinant microorganisms comprising NADPH dependent enzymes and methods of production therefor

摘要

The invention provides a recombinant carboxydotrophic Clostridia microorganism with increased overall utilization of NADPH relative to a parent microorganism. Further, the invention provides a method of producing a recombinant carboxydotrophic Clostridia microorganism which exhibits increased NADPH utilization relative to a parental microorganism. In particular, the invention relates to increasing the overall utilization of NADPH in a recombinant carboxydotrophic Clostridia microorganism in order to increase the production of at least one fermentation product by the microorganism.

主权项

1. A recombinant carboxydotrophic Clostridia microorganism adapted to express at least one exogenous NADPH-dependent enzymes or adapted to over-express at least one endogenous NADPH-dependent enzymes, the enzymes selected such that when the exogenous enzyme is expressed or the endogenous enzyme is overexpressed, the overall utilization of NADPH by the microorganism is increased relative to a parental microorganism.