HAND-HELD WIRELESS PLATFORM AND OPTICS FOR MEASUREMENT OF DNA, RNA, MICRORNAS, AND OTHER MARKERS OF PATHOGENS, GENETIC DISEASES, AND CANCER

摘要

The present invention provides compositions for making and methods of using a hand-held nucleic acid amplification device, comprising a disposable biochip with a series of sample wells, each sample well having a novel optical arrangement that includes a light-emitting diode (LED) and a single light capturing element (e.g. a photodiode) for quickly measuring light emissions from biological samples such as nucleic acid amplification reactions. Such a device can utilize isothermal amplification for obtaining detectable yields of amplified nucleic acid product in short time periods, for example, within seconds.

主权项

1. A device, comprising:
a) a plurality of sample wells, at least one of the wells containing one or more light emitting molecules; b) a plurality of Light Emitting Diodes (LEDs), each LED capable of emitting optical energy for activating the one or more of the light emitting molecules in an associated sample, wherein each of the LEDs has a vertical plane; c) a plurality of optical fibers, each of the optical fibers having first and second ends, each of the first ends separately and operably linked to an associated sample well for capturing emitted optical energy from the light emitting molecule(s), the second end configured for emitting the captured optical energy; wherein the first end is at an angle of greater than 1° and less than 90° from the vertical plane of the LED.