| 摘要 |
<p>Disclosed is a gene copy number variation measurement method. The method selects an endogenous or exogenous similar sequence similar, but not identical, to the gene sequence to be measured, designs the corresponding primers for the gene sequence to be measured and the similar sequence to amplify at the same time, and then performs a melting curve analysis. When the gene to be measured experiences copy number variations, the melting curve analysis result thereof exhibits substantial differences from the analysis result of the wild gene to be measured, thereby achieving the measurement objective. The method of the present invention is applicable not only to the measurement of gene copy number variations, but also applicable to the detection of chromosome aneuploid. The method of the present invention is not only applicable to conventional prenatal checkups and the screening for people having gene copy number variations and chromosome aneuploid, but also for non-invasive prenatal checkups.</p> |
