| 摘要 |
Whole cell catalyst expressing a transaminase and comprising an alpha -dioxygenase or a combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating fatty acid reductase, where at least one of the phosphopantetheinyl transferase and transaminase is recombinant, is new. Independent claims are included for: (1) conversion of a fatty acid, omega -hydroxy fatty acid, omega -oxo fatty acid or its monoester to an amine, comprising oxidizing a starting material comprising a fatty acid, omega -hydroxy fatty acid, omega -oxo fatty acid or its monoester by contacting the starting material with an alkane hydroxylase, and/o an alcohol dehydrogenase to obtain an oxidation product, contacting the oxidation product with the phosphopantetheinylated fatty acid reductase or the alpha -dioxygenase to obtain an aldehyde, and contacting the aldehyde with a transaminase to obtain the amine; and (2) a reaction mixture comprising the whole cell catalyst in an aqueous solution, and the fatty acid, omega -hydroxy fatty acid, omega -oxo fatty acid or its monoester. |
