MEGAKARYOCYTE PROGENITOR CELLS FOR PRODUCTION OF PLATELETS

摘要

Provided herein are methods of generating megakaryocyte lineage cells from hematopoietic stem cells in the absence of feeder cells and serum. The megakaryocyte progenitor cells (MKPs) generated as described result in rapid production of significant numbers of platelets when administered in vivo.

主权项

1. An isolated population of human cells comprising at least 40% CD34+CD41+ megakaryocyte progenitor cells (MKPs), wherein said population of cells has platelet generating activity, and wherein administration of 5×106 cells from the population of cells into an immunocompromised mouse results in at least 106 human platelets/ml blood 14 days post-administration.