发明名称 |
METHODS AND COMPOSITIONS FOR DETECTING CANCERS ASSOCIATED WITH METHYLATION OF HMLH1 PROMOTER DNA |
摘要 |
Methods are provided for detection of cancers associated with methylation of hMLH1 promoter DNA in a subject. The method comprise assaying for the presence of methylated hMLH1 promoter DNA in a bodily fluid from a subject. In one embodiment, the method comprises reacting DNA from the sample with a chemical compound that converts non-methylated cytosine bases but not methylated cytosine bases, to a different nucleotide base. The compound-converted DNA is then amplified using a methylation-sensitive polymerase chain reaction (MSP) employing primers that amplify the compound-converted DNA template. The present invention also provides nucleotide primer sequences for use in the methylation-sensitive PCR assay. |
申请公布号 |
US2014206855(A1) |
申请公布日期 |
2014.07.24 |
申请号 |
US201414157142 |
申请日期 |
2014.01.16 |
申请人 |
Case Western Reserve University |
发明人 |
Markowitz Sanford D.;Grady William |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Cleveland OH US |