| 摘要 |
PURPOSE: A primer composition for loop-mediated isothermal amplification reaction for detecting tomato yellow leaf curl virus is provided to quickly diagnose the virus using high concentration SYBR Green I under natural light with naked eye. CONSTITUTION: A primer set for loop-mediated isothermal amplification reaction for detecting tomato yellow leaf curl virus has sequence numbers 1-4. The primer set has sequence numbers 11-14. The virus is selected from the group consisting of TYLCV Jordan1 mutant strains, Jordan3 mutant strains, Israel mutant strains, Spain mutant strains, USA mutant strains, and Korea mutant strains. A primer composition for loop-mediated isothermal amplification reaction for detecting the virus contains the primer set, DNA polymerase, dNTPs, and a reaction buffer. |
