发明名称 |
Cutinase-producing genetically engineered microorganism and use thereof |
摘要 |
The present invention relates to the field of bioengineering and discloses a cutinase-producing genetically engineered microorganism and use thereof. Recombinant plasmid Tfu_0883-hlyAs/pET20b(+) was constructed and transformed into E. coli BL21(DE3) to obtain recombinant E. coli strain Tfu_0883-hlyAs/pET20b(+)/E. coli BL21(DE3). Specific growth rate was maintained at a certain value using fed-batch fermentation mode. After fermenting 30-34 hours, the enzyme activity in the supernatant reached 700-750 U/mL. The present invention uses glycerol as the main raw material and employs semi-synthetic medium, has the advantages of good stability and ease of control, and is suitable for large-scale production. |
申请公布号 |
US8785169(B2) |
申请公布日期 |
2014.07.22 |
申请号 |
US201113264560 |
申请日期 |
2011.02.28 |
申请人 |
Jiangnan University |
发明人 |
Chen Jian;Wu Jing;Wu Dan;Wang Lei |
分类号 |
C12N9/18;C12N1/20 |
主分类号 |
C12N9/18 |
代理机构 |
The Webb Law Firm |
代理人 |
The Webb Law Firm |
主权项 |
1. A cutinase-producing genetically engineered microorganism, comprising cutinase Tfu—0883 gene and hlyAs gene introduced into E. coli. |
地址 |
Wuxi CN |