Metal nanoparticle structures for enhancing fluorescence-based assays

摘要

Provided, among other things, is a multiplex assay comprising: conducting a fluorescence-developing assay on microtabs having at least one surface that shows plasmonic enhancement, wherein a plurality of the microtabs have unique probes affixed to their plasmonically enhanced surfaces; and measuring the fluorescence associated with the substrates and identifying the correlated probe by for the microtab. The microtabs can be, for example, MTPs that send a unique identifier, and the correlated probe can be identified by querying the MTPs for their identifier.

主权项

1. A fluorescence enhancing substrate configured to covalently attach assay reagent comprising:
a substrate; a first coating comprising AS and ES (AS/ES) on the substrate, wherein the coating is thicker than monolayer; and metal particles deposited or formed on the AS/ES coating, said particles effective to enhance a surface-mediated fluorescence assay by 5-fold or more, wherein AS is a reactive amine that is not a tertiary amine, and wherein the substrate with its first coating and any additional AS/ES coating(s) has amine and hydroxy functionality from AS and ES available for attaching an assay useful amount of the assay reagent.