发明名称 LOW SEQUENCE BIAS SINGLE-STRANDED DNA LIGATION
摘要 The invention provides compositions and methods for ligating single stranded nucleic acids wherein the ligation is based on fast, efficient, and low-sequence bias hybridization of an acceptor molecule with a donor molecule. In one embodiment, the structure of the donor molecule comprises a stem-loop intramolecular nucleotide base pairing (i.e., hairpin) and a 3 '-overhang region such that the overhang is able to hybridize to nucleotides present in the 3' end of the acceptor molecule.
申请公布号 WO2014110272(A1) 申请公布日期 2014.07.17
申请号 WO2014US10894 申请日期 2014.01.09
申请人 THE PENN STATE RESEARCH FOUNDATION 发明人 BEVILACQUA, PHILIP, C.;ASSMANN, SARAH, M.;DING, YILIANG;KWOK, CHUN, KIT
分类号 C12P19/34;C12N15/64 主分类号 C12P19/34
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