发明名称 |
ELECTRON-MICROSCOPIC EXAMINATION METHOD FOR EXAMINING A LIVING BIOLOGICAL SAMPLE |
摘要 |
Provided is an observation method by an electron microscope, in which a biological sample can be observed as it is alive and a situation that the biological sample is moving can be observed using an electron microscope, and a composition for evaporation suppression under vacuum, a scanning electron microscope, and a transmission electron microscope used in the method. The sample observation method by an electron microscope according to the invention includes applying a composition for evaporation suppression containing at least one kind selected from an amphiphilic compound, oils and fats, and an ionic liquid to the surface of a sample to form a thin film, and covering the sample with the thin film, and displaying an electron microscopic image of the sample, which is covered with the thin film and accommodated in a sample chamber under vacuum, on a display device. |
申请公布号 |
EP2755225(A1) |
申请公布日期 |
2014.07.16 |
申请号 |
EP20120829935 |
申请日期 |
2012.09.07 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY |
发明人 |
HARIYAMA, TAKAHIKO;TAKAKU, YASUHARU;SUZUKI, HIROSHI;MURANAKA, YOSHINORI;OHTA, ISAO;SHIMOMURA, MASATSUGU;ISHII, DAISUKE |
分类号 |
H01J37/20;G01N1/28;G01N23/04;G01N23/225;H01J37/18 |
主分类号 |
H01J37/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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