Combinations of proteins to enhance viability of stem cells and their progenitors before transplantation

摘要

Embodiments of the present invention include the use of placental alkaline phosphatase alone or in combination with human transferrin and, optionally, human α1-antitrypsin to enhance the proliferation and survival of transplanted stem cells and stem cell-derived progenitor cells.

主权项

1. A method for stimulating proliferation and promoting survival of mesenchymal stem cells, or hematopoietic stem cells, or their progenitor cells before transplantation, the method comprising the steps of (i) contacting the mesenchymal stem cells, or hematopoietic stem cells, or their progenitor cells with a first composition comprising 1-50 μg per ml of an active placental alkaline phosphatase or its variant in a suitable cell culture medium containing 0-10% serum, and (ii) harvesting said cells for transplantation in a suitable medium supplemented with a second composition comprising an active placental alkaline phosphatase or its variant; wherein said active placental alkaline phosphatase or its variant comprises a shortened and/or altered placental alkaline phosphatase sequence and has cell proliferation stimulation activity.