| 摘要 |
The invention relates to an apparatus for transmitted light illumination for light microscopes, in particular stereo microscopes or macroscopes, having changing effective entrance pupil of an objective. The apparatus has a light source for emitting an illuminating light beam, a housing, in which the light source is arranged, a holding device for holding a sample to be examined and at least one diaphragm edge for trimming the illuminating light beam, wherein the diaphragm edge extends transversely to an optical axis of a light microscope, which can be positioned in an operating state on the device for transmitted light illumination, and wherein a beam path of the illuminating light between the diaphragm edge and the sample held by the holding device is free from adjustable beam focussing components. The apparatus is characterised according to the invention in that in order to adapt the beam path of the illuminating light to the effective entrance pupil of the objective, means are provided for variable positioning of the diaphragm edge in the direction of the optical axis, wherein a position of the diaphragm edge in the direction of the optical axis can be varied irrespectively of a position of the diaphragm edge transversely to the optical axis, assembly means are provided on the housing for mounting a separate sample support able, means for determining the presence of a sample support table are present, the housing has a passage opening, through which the diaphragm edge can be moved in the direction of the optical axis, the holding device is formed in the region of the passage opening of the housing or on a separate sample support table and a control device is present which is adapted to position the diaphragm edge in dependence at least upon a determined presence of a sample support table. The invention also relates to a method for transmitted light illumination for a light microscope and a microscope system. |
