| 摘要 |
The present invention relates to: a novel brazzein multiple variant of increased sweetness; a nucleic acid molecule coated with the variant; a recombinant vector comprising the nucleic acid molecule; host cells that have been genetically altered by means of the recombinant vector; a method for making the variant; and a food composition for increasing sugar levels, which comprises the variant as an active ingredient. The brazzein multiple variant of the present invention is at least about two million times as sweet as sucrose (sugar) in the same amount, and the sweetness increase is far beyond even wild brazzein and brazzein variants that have previously been developed. Consequently, the brazzein variant of the present invention can be used in a very small amount to bring out the desired sweetness, and can be used to replace sugar or other sweeteners in food. |
