Methods for making nucleotide probes for sequencing and synthesis

摘要

Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.

主权项

1. A method of making a plurality of probes for analyzing a plurality of nucleic acid samples comprising the steps of:
providing linear, single stranded DNA encoding a plurality of probes, wherein a probe includes two regions of homology to target genomic DNA at the ends of the probe and two removable PCR primer regions common to all probes; converting the linear, single stranded DNA to circular DNA; amplifying the circular DNA; releasing the plurality of probes from the amplified DNA; and removing the removable PCR primer regions from the probes.