Method of identifying target biomolecule by using probe-binding frequency

摘要

An efficient and accurate method of identifying a target biomolecule in a sample by using target molecule-probe binding frequencies is disclosed.

主权项

1. A method of identifying a target biomolecule, the method comprising:
detecting the binding of each of N nucleic acid probe molecules to a target biomolecule to generate a target signature, which is defined by the binding frequencies of the individual N nucleic acid probe molecules to the target biomolecule, wherein N≧2 and each nucleic acid probe molecule has a unique configuration element that permits specific binding of the nucleic acid probe molecule to the target molecule, wherein the target biomolecule is a nucleic acid; comparing the target signature to a reference signature pool, wherein the reference signature pool comprises a reference signature for each of a plurality of reference biomolecules, and each reference signature is defined by the binding frequencies of the individual N nucleic acid probe molecules to a reference biomolecule; and identifying the target biomolecule as being identical to a reference biomolecule if the binding frequencies of the individual nucleic acid probe molecules in the target signature match those in the reference signature within a predetermined confidence level of about 95% or greater with respect to the N nucleic acid probe molecules, wherein identifying the target biomolecule further comprises: calculating a binding frequency distance (d) according to Equation (1) below, wherein xi denotes an ith binding frequency of the target signature, and yi denotes an ith binding frequency of each of the reference signatures in the reference signature pool;d⁡(x→,y→)=∑i=1N⁢(xi-yi)2Equation⁢⁢1 and generating a target-reference distance distribution of binding frequency distance (d) defined by a number of counts with respect to each of the binding frequency distances (d), wherein the target biomolecule is identified as being identical to one of the reference biomolecules having a binding frequency distance (d) that is smaller or equal to a predetermined threshold in the target-reference distance distribution.