APPARATUS AND METHOD FOR IDENTIFYING A HOOK EFFECT AND EXPANDING THE DYNAMIC RANGE IN POINT OF CARE IMMUNOASSAYS

摘要

The present invention relates to systems and methods for the rapid in situ determination of the existence of a hook effect and expansion of the dynamic range of a point of care immunoassay. For example, a system for identifying a hook effect and expanding the dynamic range of an immunoassay is provided that may include a primary sensor having first immobilized antibodies that may be configured to generate a first signal based on a presence or absence of a target analyte in a sample. The system may further include an attenuated sensor having second immobilized antibodies at a reduced concentration relative to a concentration of the first immobilized antibodies on the primary sensor and that may be configured to generate a second signal based on the presence or absence of the target analyte in the sample. The system may further include a processor configured to determine a presence of a hook effect in the immunoassay based on relative values of the first and second signals and optionally determine the target analyte concentration of the sample.

主权项

1. A system for identifying a hook effect, the system comprising:
a first capture reagent comprising an antigen-binding reagent configured to generate a first signal based on a presence or absence of a target analyte in a sample; a second capture reagent comprising an attenuated antigen-binding reagent at a reduced concentration relative to a concentration of the antigen-binding reagent and configured to generate a second signal based on the presence or absence of the target analyte in the sample; and a processor configured to determine a presence of a hook effect in an assay based on relative values of the first and second signals.