ANTIBODY-BASED ARRAYS FOR DETECTING MULTIPLE SIGNAL TRANSDUCERS IN RARE CIRCULATING CELLS

摘要

The present invention provides antibody-based arrays for detecting the activation state and/or total amount of a plurality of signal transduction molecules in rare circulating cells and methods of use thereof for facilitating cancer prognosis and diagnosis and the design of personalized, targeted therapies.

主权项

1. A kit for performing an antibody-based array method, the kit comprising:
(a) a dilution series of a plurality of capture antibodies restrained on a solid support; and (b) a plurality of detection antibodies comprising activation state-independent antibodies and activation state-dependent antibodies; and (c) instructions for methods of using the kit to detect the activation states of a plurality of signal transduction molecules of circulating cells of a solid tumor.