<p>A method for detecting a methylated genomic locus is provided. In certain embodiments, the method comprises: a) treating a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an agent that modifies cytosine to uracil to produce a treated nucleic acid; b) amplifying a product from the treated nucleic acid using a first primer and a second primer, wherein the first primer hybridizes to a site in the locus that contain methylcytosines and the amplifying preferentially amplifies the methylated copies of the genomic locus, to produce an amplified sample; and c) detecting the presence of amplified methylated copies of the genomic locus in the amplified sample using a flap assay that employs an invasive oligonucleotide having a 3' terminal G or C nucleotide that corresponds to a site of methylation in the genomic locus.</p>
申请公布号
AU2011329375(B2)
申请公布日期
2014.07.03
申请号
AU20110329375
申请日期
2011.11.02
申请人
EXACT SCIENCES CORPORATION
发明人
ZOU, HONGZHI;LIDGARD, GRAHAM P.;DOMANICO, MICHAEL J.;ALLAWI, HATIM