ENDONUCLEASE-ENHANCED HELICASE-DEPENDENT AMPLIFICATION

摘要

The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.

主权项

1. A composition comprising a restriction endonuclease, a helicase, a DNA polymerase, and at least a first primer and a second primer,
wherein the restriction endonuclease is one which cannot recognize or cleave the amplification product which is generated in the presence of the composition and a nucleic acid which hybridizes to the at least first and second primer.