| 发明名称 | DNA polymerases with increased 3′-mismatch discrimination | ||
| 摘要 | Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | ||
| 申请公布号 | US8765435(B2) | 申请公布日期 | 2014.07.01 |
| 申请号 | US201113180198 | 申请日期 | 2011.07.11 |
| 申请人 | Roche Molecular Systems, Inc. | 发明人 | Bauer Keith;Reichert Fred;Myers Thomas W. |
| 分类号 | C12N9/00;C12N15/54 | 主分类号 | C12N9/00 |
| 代理机构 | Kilpatrick Townsend & Stockton LLP | 代理人 | Kilpatrick Townsend & Stockton LLP |
| 主权项 | 1. A thermostable, family A-type DNA polymerase having increased 3′-mismatch discrimination activity compared with a control DNA polymerase, wherein the DNA polymerase comprises a motif in the polymerase domain comprising V-D-P-L-M-R—R-A-X6—K-T-V—N—F, wherein: X6 is any amino acid other than A or G (SEQ ID NO: 10); wherein the DNA polymerase has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:1, 2, 3, 4, 5, 6, or 7, and wherein the control DNA polymerase has the same amino acid sequence as the DNA polymerase except that the amino acid of the control DNA polymerase at position X6 is A or G. | ||
| 地址 | Pleasanton CA US | ||