Methods and compositions for improving sugar transport, mixed sugar fermentation, and production of biofuels

摘要

The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell, methods of increasing transport of a pentose into a cell, methods of increasing growth of a cell on a medium containing pentose sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell.

主权项

1. A method of increasing transport of cellodextrin into a cell, comprising:
culturing a host cell which comprises a recombinant polynucleotide encoding a cellodextrin transporter polypeptide in a medium such that the recombinant polynucleotide is expressed, said cellodextrin transporter having the structure of a Major Facilitator Superfamily protein and comprising transmembrane α-helix 1, α-helix 2, α-helix 3, α-helix 4, α-helix 5, α-helix 6, α-helix 7, α-helix 8, α-helix 9, α-helix 10, α-helix 11, α-helix 12, said transmembrane α-helix 2 characterized by:
a tyrosine or phenylalanine at the position corresponding to amino acid 1 of SEQ ID NO:2;a glycine at the position corresponding to amino acid 4 of SEQ ID NO:2;a proline, valine, or phenylalanine at the position corresponding to amino acid 10 of SEQ ID NO:2; andan aspartate or glutamine at the position corresponding to amino acid 17 of SEQ ID NO:2, wherein expression of the recombinant polynucleotide results in increased transport of cellodextrin into the cell compared with a cell that does not comprise the recombinant polynucleotide.