Engineered E2 for increasing the content of free LYS11-linked ubiquitin

摘要

The invention provides a chimeric E2 enzyme comprising a Ubc domain fused to a heterologous ubiquitin binding domain (UBD). The chimeric enzymes of the invention may be useful in producing elevated levels of free polyubiquitin.

主权项

1. A chimeric enzyme comprising a UBE2S ubiquitin conjugating enzyme (E2) having a ubiquitin conjugating (Ubc) domain, from which an N-terminal tail or a C-terminal tail has been removed, fused to a heterologous ubiquitin-binding domain (UBD).