Method of generating an optimized, diverse population of variants

摘要

The disclosure relates to a method of generating a diverse set of variants to screen improved and novel properties within the variant population, a system for creating the diverse set of variants, and the variant peptides.

主权项

1. A method for generating a maximally diverse population of molecular variants, the method comprising the steps of:
(a) inputting a desired set of mutations; (b) setting optimization parameters, wherein the optimization parameters comprise:
(i) number nvar of molecular variants to create;(ii) molecular population size popSize;(iii) a crossover rate;(iv) a mutation rate;(v) repair operator;(vi) a primary fitness function; and(vii) a penalty fitness function; (c) generating a plurality of random genomes of population size popSize; (d) creating a first generation of genomes of the size nvar by applying a selection operator; a crossover operator; a mutation operator; a repair operator; a primary fitness function operator; and penalty function operator on the plurality of random genomes,wherein operations (a)-(d) are performed by executing instructions on a computer system programmed to perform said operations.