| 摘要 |
Disclosed is a method for producing a cell line that expresses a protein of interest or an RNA of interest, wherein the cell line has at least one desired characteristic that is consistent over time, comprising the steps of: a) providing a plurality of cells that express mRNA encoding the protein of interest or the RNA of interest; b) dispersing cells individually into individual culture vessels, thereby providing a plurality of separate cell cultures; c) culturing the cells under a set of desired culture conditions using automated cell culture methods characterized in that the conditions are substantially identical for each of the separate cell cultures, during which culturing the number of cells per separate cell culture is normalized from culture to culture, and wherein the separate cultures are passaged on the same schedule; d) assaying the separate cell cultures for the at least one desired characteristic at least twice; and e) identifying a separate cell culture that has the at least one desired characteristic in the assays of step d) and expanding said identified cell culture into a cell line. |
