| 摘要 |
Disclosed is a method of transfecting a non-human cell comprising introducing into the cell: an exogenous nucleic acid and a nucleoprotein filament of a proteinaceous fusion molecule and a nucleic acid probe complementary to a target site of DNA of the cell, wherein the fusion protein comprises a recombinase domain that contributes to the filament, a protein-protein interaction domain, and a nuclear localization signal domain, wherein the exogenous nucleic acid is incorporated into the DNA of the cell and expressed by the cell. Further disclosed is a purified composition for transfection of exogenous DNA into chromosomal DNA of a non-human cell, the composition comprising a nucleoprotein filament of a probe and a proteinaceous fusion molecule, wherein the probe comprises double-stranded denatured DNA complementary to a chromosomal DNA site, and the fusion molecule comprises a recombinase domain and a protein-protein interaction domain, wherein the composition is free of DNA sequences that specifically bind to the protein-protein interaction domain. |
