NEUROGENESIS SCREENING METHOD AND USES THEREOF

摘要

Provided herein are methods for detecting and/or confirming the neurogenesis effect of eicosapentaenoic acid utilizing adipose-derived stem cells (ADSCs). Further provided are methods of promoting neurogenesis in ADSCs. Also provided are methods for the use of eicosapentaenoic acid for the production of a neurologic component that may be incorporated into a nutritional composition for promoting neurogenesis in a pediatric subject.

主权项

1. A method for detecting neurogenesis of eicosapentaenoic acid, comprising:
a) culturing adipose-derived stem cells (ADSCs) in the presence of eicosapentaenoic acid; and determining the extent of neurogenesis in the ADSCs cultured in the presence of eicosapentaenoic acid; b) culturing ADSCs in the absence of eicosapentaenoic acid and determining the extent of neurogenesis in the ADSCs cultured in the absence of eicosapentaenoic acid; and c) comparing the extent of neurogenesis in the ADSCs cultured in the presence of eicosapentaenoic acid to the extent of neurogenesis of the ADSCs cultured in the absence of eicosapentaenoic acid, and d) determining the extent of neurogenesis by observing a change in cell morphology of the ADSCs, wherein an increase in the extent of neurogenesis in the ADSCs cultured in the presence of eicosapentaenoic acid compared to the extent of neurogenesis in the ADSCs cultured in the absence of eicosapentaenoic acid indicates that the candidate compound is a neurogenesis-promoting compound.