Method of Reseeding Adherent Cells Grown in a Hollow Fiber Bioreactor System

摘要

This invention is directed to methods of directly reseeding harvested adherent cells grown in a hollow fiber bioreactor. Also disclosed is a novel harvest media for use in directly reseeding adherent cells into a hollow fiber bioreactor.

主权项

1. A method for directly reseeding harvested adherent cells grown in growth media in a hollow fiber bioreactor having an intracapillary space and an extracapillary space wherein the cell growth space is one of the intracapillary space or the extracapillary space, comprising the steps of:
removing the growth media from the cell growth space; washing the cells to remove residual growth media from the cell growth space; loosening the cells from the cell growth space by adding trypsin to the cell growth space; removing the cells and any trypsin from the cell growth space; inactivating the trypsin from the removed cells; and directly reseeding the removed cells and trypsin into the cell growth space of a hollow fiber bioreactor.