BUFFER KIT AND METHOD OF GENERATING A LINEAR pH GRADIENT

摘要

A buffer kit includes a first eluent and second eluent. The first eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The second eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The first eluent solution has a first pH and the second eluent solution has a second pH where the first pH and second pH are different values. The buffer kit provides a linear pH gradient that forms an approximately straight line from at least the first pH to the second pH.

主权项

1. A buffer kit comprising:
a) a first eluent solution including at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge over a pH range of about 6 to about 10, and include a sulfonate group and an amine, where the first eluent solution has a first pH of about 6 and a total buffer salt concentration of greater than about 25 millimolar; b) a second eluent solution including at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge over a pH range of about 6 to about 10, and include a sulfonate group and an amine, where the second eluent solution has a second pH of about 10 and a total buffer salt concentration of greater than about 25 millimolar; whereby the buffer kit provides a linear pH gradient, based on a function of time and pH values, that forms an approximately straight line for at least a pH range of about pH 6 to about pH 10.