TOTAL POLYSACCHARIDES OF RADIX ISATIDIS AND THEIR FRACTIONS, AND USES THEREOF AS VACCINE ADJUVANTS

摘要

The present invention pertains to medicinal technical field, relates to a Radix isatidis total polysaccharide and fractions thereof and their uses as vaccine adjuvant. Specifically, it relates to a Radix isatidis total polysaccharide as well as neutral polysaccharide fraction and acidic polysaccharide fraction extracted from Chinese medicinal material Radix isatidis, and to their uses as vaccine adjuvant or uses in manufacture of vaccine composition. The present invention further relates to a vaccine adjuvant and vaccine preparation comprising the above Radix isatidis total polysaccharide or polysaccharide fraction, a method for preparing antibody, and a method for immunization or inoculation. The Radix isatidis total polysaccharide as well as neutral polysaccharide fraction and acidic polysaccharide fraction of the present invention all can be used as adjuvant for attenuated vaccines, protein vaccines, DNA vaccines or polypeptide vaccines.

主权项

1. A Radix isatidis polysaccharide fraction having the following characteristics:
(1) which comprises glucose, galactose, mannose, rhamnose, arabinose and xylose, and has molar ratio of Rha:Ara:Xyl:Man:Glc:Gal=1.00:2.35:2.38:9.27:27.47:13.03; (2) expressed in glucose, saccharide content is 98.13%; (3) molecular weight is 2000-10000.