| 摘要 |
<p>The present disclosure relates to a method for identifying a cell producing an antibody specific to a target antigen comprising the steps of: a) providing a population of antibody producing B cells, wherein said population has been selected on the basis of at least one B cell marker and/or at least one marker selected from the group consisting of an IgG, IgE and IgA marker, and two or more "de-selection" markers (not present on the target B cell), b) incubating the population of B cells from step a) with said target antigen and at least two distinct labels for said antigen such that the antigen is in a form labelled with a first label and/or a second label, c) screening the cell population for those cells capable of producing antibodies specific to the target antigen wherein the cells of interest are identified by at least one of each of the two or more distinct labels, and d) selecting the multi-antigen-labelled population of cells identified from step c), and e) optionally separating the population of cells selected in step d) into single cells.</p> |
