CONSTRUCTS AND STRAINS FOR FIXING CARBON DIOXIDE AND METHODS FOR PREPARING THE SAME

摘要

The present invention relates to a construct for accomplishing fixation of carbon dioxide and/or reduction of carbon dioxide emission in a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli), a vector comprising the construct, a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli) comprising the construct or being transformed with the vector, and a method for fixing carbon dioxide and/or reducing carbon dioxide emission in a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli).

主权项

1. A microorganism comprising:
a first gene; and a second gene; wherein the first gene is selected from the group consisting of:
1) a phosphoribulokinase (Prk) gene (EC2.7.1.19);2) a nucleotide sequence that has at least 80% identity to the sequence of a phosphoribulokinase (Prk) gene (EC2.7.1.19); and3) a nucleotide sequence capable of hybridizing with the sequence of a phosphoribulokinase (Prk) gene (EC2.7.1.19) under stringent hybridization conditions; and wherein the second gene is selected from the group consisting of:
4) a Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) gene (EC 4.1.1.39);5) a nucleotide sequence that has at least 80% identity to the sequences of a Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) gene (EC 4.1.1.39); and6) a nucleotide sequence capable of hybridizing with the sequences of a Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) gene (EC 4.1.1.39) under stringent hybridization conditions;wherein the microorganism is a heterophic microorganism.