主权项 |
1. A process for preparing a purified conjugate in a solution, wherein the conjugate comprises an effector or a reporter molecule linked to a cell binding agent, the process comprising the steps of:
(a) contacting the effector or the reporter molecule with a bifunctional linker reagent to covalently attach the linker to the effector or the reporter molecule and thereby prepare an unpurified first mixture comprising the effector or the reporter molecule having linkers bound thereto, (b) conjugating a cell binding agent to the effector or the reporter molecule having linkers bound thereto by reacting the unpurified first mixture with the cell binding agent to prepare a second mixture, and (c) subjecting the second mixture to tangential flow filtration, dialysis, gel filtration, adsorptive chromatography, selective precipitation or a combination thereof to thereby prepare the purified conjugate. |