发明名称 |
DETECTION OF IMMUNOGLOBULIN LIGHT CHAIN RESTRICTION BY RNA IN SITU HYBRIDIZATION |
摘要 |
The invention provides a method for detecting immunoglobulin light chain restriction and clonality in B cells by obtaining a sample of B cells from a subject; conducting a duplex in situ hybridization assay on the sample using (i) at least one probe set which is designed to specifically hybridize to immunoglobulin kappa chain constant region (IGKCR) RNA; and (ii) at least one probe set which is designed to specifically hybridize to immunoglobulin lambda chain constant region (IGLCR) RNA; detecting signal associated with hybridized IGKCR probe and signal associated with hybridized IGLCR probe in a population of B cells in the sample; and determining a pattern of signal associated with hybridized IGKCR probe and hybridized IGLCR probe within individual cells in the B cell population, wherein the pattern of signal within individual cells indicates the presence or absence of light chain restriction and clonality of the B cells. |
申请公布号 |
US2014178869(A1) |
申请公布日期 |
2014.06.26 |
申请号 |
US201313843408 |
申请日期 |
2013.03.15 |
申请人 |
MA Xiao-Jun;LUO Yuling;Tubbs Raymond |
发明人 |
MA Xiao-Jun;LUO Yuling;Tubbs Raymond |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A method for detecting immunoglobulin light chain restriction and clonality in B cells, the method comprising:
(a) obtaining a sample of B cells from a subject; (b) conducting a duplex in situ hybridization assay on the sample using (i) at least one probe set which is designed to specifically hybridize to immunoglobulin kappa chain constant region (IGKCR) RNA; and (ii) at least one probe set which is designed to specifically hybridize to immunoglobulin lambda chain constant region (IGLCR) RNA; (c) detecting signal associated with hybridized IGKCR probe and signal associated with hybridized IGLCR probe in a population of B cells in the sample; and (d) determining a pattern of signal associated with hybridized IGKCR probe and hybridized IGLCR probe within individual cells in the B cell population, wherein the pattern of signal within individual cells indicates the presence or absence of light chain restriction and clonality of the B cells. |
地址 |
Pleasanton CA US |