<p>A method of wound prognosis comprising the steps of: determining if the level of human neutrophil elastase (hNE) in a sample of wound fluid from a wound exceeds a first predetermined threshold; determining if the level of at least one matrix metalloproteinase (MMP) in a sample of wound fluid from said wound exceeds a second predetermined threshold; and assigning said wound to a non-healing category if either said level of hNE or said level of MMP exceeds said first or second threshold, respectively. Also provided are devices, such as lateral flow devices, for performing the method.</p>