摘要 |
A method of producing squalene and ethanol using yeast, in particular Saccharomyces cerevisiae, is described. The squalene epoxidase gene ERG1 is placed under the control of a tetracycline-responsive promoter, and exposure to doxycycline is used to suppress gene expression and allow accumulation of squalene. The process is adapted for use with agricultural feedstocks, in one embodiment using a grass juice extracted from ryegrass. |