In situ expression of lipase for enzymatic production of alcohol esters during fermentation

摘要

Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.

主权项

1. A method comprising:
(a) providing a fermentation medium comprising fermentable carbon substrate derived from a biomass feedstock and an alcohol-producing yeast microorganism wherein the alcohol-producing microorganism comprises an engineered polynucleotide encoding a polypeptide having lipase activity and the microorganism expresses and displays or secretes said polypeptide such that the lipase activity is present in the fermentation medium, wherein the alcohol-producing microorganism further comprises reduced or eliminated pyruvate decarboxylase activity, and wherein the microorganism ferments the fermentable carbon substrate to produce alcohol, wherein the alcohol is a C3 to C8 alkyl alcohol; and (b) contacting the fermentation medium with a carboxylic acid wherein the lipase activity is present in the fermentation medium in sufficient amount to convert at least a portion of the alcohol produced by the microorganism to alcohol esters extracellularly.