Temperature control device with a flexible temperature control surface

摘要

A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.

主权项

1. A temperature-control bladder assembly for performing a polymerase chain reaction (PCR), comprising:
a top bracket comprising a first temperature-control bladder; a bottom bracket comprising a second temperature-control bladder; and a middle bracket that, when assembled with said top bracket and bottom bracket, defines an interior space, wherein said first and second bladders form contacts with a reaction chamber placed inside said interior space when inflated with a temperature-control fluid, wherein said reaction chamber is in the form of a PCR tube or a microarray slide.