Type I polyketide synthase extender units

摘要

Novel extender units for Type I polyketide synthases are provided. Also provided are genes, compounds, and methods for generating these units, and for incorporation of the novel extender units into polyketides for the purpose of generating new structural derivatives of polyketide-containing products.

主权项

1. A method for incorporating an aminomalonyl into a hybrid polyketide molecule, comprising adding and reacting aminomalonyl-acyl carrier protein (AM-ACP) in a cell-free system with
(a) an isolated polypeptide comprising an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO:6 and an amino acid sequence that is at least 95% identical to SEQ ID NO:6, wherein said isolated polypeptide has the biological activity of catalyzing the incorporation of an ethanolamine subunit from AM-ACP into a polyketide; and (b) a second isolated polypeptide comprising (i) an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO:7 and an amino acid sequence that is at least 95% identical to SEQ ID NO:7, whereby the second isolated polypeptide has ketosynthase activity, (ii) an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO:8, whereby the second isolated polypeptide has ketoreductase activity, and (iii) an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO:9 and an amino acid sequence that is at least 95% identical to SEQ ID NO:9, whereby the second isolated polypeptide has acyl carrier protein activity, wherein said isolated polypeptides of (a) and (b) incorporate the aminomalonyl into the hybrid polyketide molecule.