CHEMICAL LIGATION

摘要

Methods comprising chemical ligation of oligonucleotides are provided. In some embodiments, methods of detecting a polymorphisms in nucleic acids are provided. In some embodiments, methods of detecting at least one analyte are provided. In some embodiments, methods of labeling solid support particles are provided. Kits comprising oligonucleotides with chemically ligatable moieties are also provided.

主权项

1. A method of detecting a single nucleotide polymorphism in a target nucleic acid, comprising:
(a) contacting said target nucleic acid with a first allele-specific primer that hybridizes to a portion of the target nucleic acid comprising the single nucleotide polymorphism and a locus-specific primer, wherein the first allele-specific primer comprises a 3′ nucleophile, and the locus-specific primer comprises a 5′ leaving group, wherein the first allele specific primer and the locus-specific primer hybridize to the target nucleic acid such that the 5′ end of the locus-specific primer is adjacent to the 3′ end of the first allele-specific primer, under conditions allowing chemical ligation between the first allele-specific primer and the first locus-specific primer to form a ligated product; and (b) detecting the ligated product.